The charge in the nucleic acids is proportional to the size of the nucleic acid sequence. Electrophoresis separates the RNA molecules based on the charge of the nucleic acids. PrincipleĪs all normal blotting technique, northern blotting starts with the electrophoresis to separate RNA samples by size. The primary difference between these two techniques is that northern blotting concerns only about RNA. The technique got its name due to the similarity of the process with Southern blotting. The northern blot technique was developed in 1977 by James Alwine, David Kemp and George Stank at Stanford University. It also aids in the identification of abnormal, diseased or infected condition at the molecular level. In different stages of differentiation and morphogenesis the abundance of an RNA changes and this can be identified using this technique.

This blotting technique can also be used for the growth of a tissue or organism. This method reveals the identity, number, activity, and size of the particular gene. mRNA is generally represented as 5% of the overall RNA sequence.

It is done by detection of particular RNA (or isolated mRNA). Northern Blotting is a technique used for the study of gene expression. Hybridization or identification of the molecule.Transferring the molecules to a nitro cellulosic membrane/ nylon membrane.Separation of the molecule of interest by an electrophoresis membrane.Finally, the coded mRNA produces the concerned protein, this protein identification can be done by Western Blotting. During gene expression, when the DNA is expressed as mRNA for a protein production, this process can be identified by Northern blotting. When a DNA sequence is the foundation or code for a protein molecule, the particular DNA molecule of interest can be blotted using Southern Blotting technique. Subtypes of blotting such as northern, western & southern depend upon the target molecule that is being sought. Protein synthesis involves expression of a DNA segment which gets converted to mRNA to produce the respective protein. The blotting technique is a tool used in the identification of biomolecules such ad DNA, mRNA and protein during different stages of gene expression. It uses hybridization techniques for the identification of the specific nucleic acids and genes. This technique immobilizes the molecule of interest on a support, which is a nitrocellulosic membrane or nylon. 1996 5(5):675-684.Blotting is used in molecular biology for the identification of proteins and nucleic acids and is widely used for diagnostic purposes. Theodosiou AM et al.'A member of the MAP kinase phosphatase gene family in mouse containing a complex trinucleotide repeat in the coding region'.Centromeric polymerase III transcription units in Chironomus pallidivittatus. cdc2MsB, a cognate cdc2 gene from alfalfa, complements the G1/S but not the G2/M transition of budding yeast cdc28 mutants. A Novel Drosophila Minute Locus Encodes Ribosomal Protein S13. Isolation of RNA from Floral Tissue of Rumex acetosa (Sorrel). Cloning and expression of a rat brain L-glutamate transporter. Direct mRNA Isolation Using Magnetic Oligo (dT) Beads: A Protocol for All Types of Cell Cultures, Animal and Plant Tissues. This is particularly important when working with small and precious samples or when isolating low-abundance transcripts. Using a solid-phase enables all procedures to be conducted in a single tube and helps ensure that losses caused by multiple sample manipulations are minimized. The unique paramagnetic properties of Dynabeads facilitate easy and efficient handling, whilst also eliminating the requirement for centrifugation, precipitation, and the use of hazardous chemicals. The flexible and scalable method fits any sample size, and is particularly useful for northern analysis starting from small amounts of sample material. Dynabeads Oligo(dT)25 can be regenerated and reused at least four times.ĭynabeads Oligo(dT)25 allows the isolation of mRNA directly from crude samples or from total RNA.Stringent hybridization and washing buffers along with strong RNase inhibition helps ensure isolation of full-length, intact mRNA.Easy and efficient magnetic handling minimizes losses caused by sample manipulations.The isolation protocol can be scaled up or down.Isolate mRNA from total RNA or directly from crude samples in only 15 minutes.Benefits of mRNA isolation with Dynabeads Oligo(dT)25

Northern blot results